The biomolecular and biodeterioration analysis is a set of analysis that allows to map biological growth, detect early biofilms, to determine diversity of the microbial community and to determine metabolic activity. (M1) detection - stereozoom microscope, digital microscope and portable VP-SEM-EDS with cryogenic platform. (M2) main objective- determine diversity of the microbial community. (1) DNA extraction and quantification. (incubation bath/centrifugation system /Electrophoresis system/ fluorimetry DNA quantification systemQuantus™ (Promega). (2) 16S rRNA gene-based amplicon sequencing. The microbial diversity will be determined by sequencing of 16S rRNA gene amplicons. high-throughput sequencing-based microbiome analysis by NGS system- MinIon nanopore. (M3) main objective- determine metabolic activity. by viability/metabolic activity assays. (1) ATP quantification by bioluminescence, Lumitester PD-20 luminometer. (2) Flow cytometry viability assays (Muse Cell Analyzer uses miniaturized fluorescence detection and microcapillary cytometry)
Fields of application
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Cultural heritage
archaeological object and site, architecture, art, demo anthropologic object, film, manuscript, mosaics, musical instrument, other, painting, papyrus, photo, textile
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Natural heritage
animal product, botanic collection, mineral, object in formalin, other, shell, skeleton, taxidermy collection
Materials
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inorganic
glass, stone, metal and metallurgical By-Products, ceramic (clay, mud brick, terracotta, earthenware, stoneware, porcelain), pigment
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organic
binding media, glues, wood, paper, textiles, varnishes
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organic and inorganic
TOOLS
The biomolecular and biodeterioration analysis Molab is Modular Lab that provides a set of analysis that allows: to map biological growth, detect early biofilms; to determine diversity of the microbial community and to determine metabolic activity. It is comprised of : Module 1 | detection of biofilms (M1), stereozoom microscope, digital microscope and portable VP-SEM-EDS...