SEC is a type of liquid chromatography, which enables the separation of molecules according to their size. The main characteristic of stationary phases are different sized pores. Smaller molecules enter these pores and therefore they move along the column slowly in comparison to larger molecules. Some molecules are too large to enter the pores and they are excluded and consequently have no retention.
Fields of application
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Cultural heritage
manuscript, painting, papyrus, textile
Materials
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organic
animal parts, binding media, glues, wood, paper, textiles, varnishes, dyes, inks
TOOLS
Size-Exclusion Chromatograph with refractive index detector (RefractoMax520), UV detector (RS Variable Wavelength Detector), viscometer and multi angle light scattering detectors; Termo Scientific Dionex UltiMate 3000 UHPLC.